Expression of DNMIκB significantly inhibited the Mcl 1 promoter ac tivity

There fore both staining patterns were evaluated separately to identify a possible cell specific localisation of EGFR and to evaluate an association of a specific pattern with prognostic pathological parameters. Membrane staining is defined as any immunoreaction in part of the cell membrane, while the cytoplas mic immunoreaction is confined to the intracellular compartment. A positive AS703026 代理店 membranous staining was found in 36. 4% and a cytoplasmic reac tion in 67%. Both expression patterns were significantly associated. No associations between membranous or cytoplasmic EGFR expression, and clinico pathological parameters as listed in Table 1 were found. Membranous EGFR expression is related to CRM1 and COX 2 expression To identify an association of EGFR with CRM1 and COX 2, we compared EGFR protein expression with data on CRM1 and COX 2 expression which was inves tigated previously.<br><br> The nuclear export protein CRM1 is expressed both in the nucleus and cytoplasm of ovarian carcinomas as described recently. Here, we found that membranous EGFR expression is asso ciated with nuclear CRM1 expression. Further, membranous EGFR expression is associated with COX 2 expression. AZD1152-HQPA 価格 CRM1 and COX 2 expression correlated with each other, as described pre viously. Membranous EGFR expression is an independent prognostic factor for overall survival In univariate Kaplan Meier survival analysis and logistic regression analysis, high tumor grade, prognostic factor for overall survival in ovarian cancer patients. For cytoplasmic EGFR expression, no significant differences were found.<br><br> In a subgroup analy sis, membranous EGFR expression was observed in 25 out of 80 serous ovarian carcino intraoperative residual tumor, and patient age are significantly associated with unfavourable AZD2281 763113-22-0 patient overall survival. FIGO stage, tumor and nodal stage did not reach prognostic significance in our cohort. Further, membranous EGFR expression was significantly asso ciated with shorter overall survival. In a multivariate Cox regression analysis adjusted for other prognostic factors, membranous EGFR expression was confirmed as an independent mas and significantly related to poor overall survival in this cancer subtype, which was confirmed in a multivariate analysis. No association between mem branous EGFR and non serous cancer type was observed.<br><br> Regarding the progression free sur vival, no significant differences for membranous or cyto plasmic EGFR expression were found. Since expression data of membranous EGFR was strongly related to CRM1 and COX 2 expression levels, we aimed to evaluate the impact of a combination of these molecules on patient survival. Here, we observed that nuclear CRM1 as well as COX 2 expression is related to poor overall survival in ovarian carcinomas, which is in line with previous findings. Combined expression of mEGFR COX 2 was significantly associated with unfavorable overall sur vival in all carcinomas and the serous sub type. In addition, combined mEGFR COX 2 expression was an independent prognostic factor in multivariate analysis adjusted for other prognostic factors. Com bined mEGFR CRM1 showed a trend for unfavorable overall survival in all carcinomas. The combination of EGFR COX 2 CRM1 had no significant impact on overall survival.