No such effect was detectable in MCF 7 cells treated in a similar manner in ind

work software was used to visualize the expression levels in each array. The color plots derived from each array are given in Additional file 2, Figure KU-0063794 mTOR 阻害剤 S1, S2, S3 and the respective Figure legends in Additional File 1. Briefly, cDNA microarray measurements are made using the two color fluorophores Cy3 and Cy5, where one color corresponds to the control and the other to the sample of interest. Dye swapping method was used to minimize the effects of the differential incorporation of these two dyes into the cDNAs derived from the mRNAs. The measured values are reported as the loga rithm base 2 of the ratio of the two chan nels. The input data of the color plots in Additional File 2 figures S1, S2 and S3 are. txt files containing the log2 ratio of medians of the intensity signals from trea ted and untreated samples.<br><br> These were calculated by the Acuity software from Lenalidomide TNF-alpha 受容体 阻害剤 the low ess normalized. gpr files obtained from scanning the microarrays by Gene Pix, Genes flagged absent or flagged bad were excluded from the calculations. Array statistical analysis and pattern recognition, after acquisition by using GenePix6 software the. gpr data generated were loaded onto the Acuity software for lowess normalization and T test statistical analysis. Four arrays for FTI, five arrays for ram1 and eight arrays for GGTI 298 were used for statistical analysis. Only the spot signals that were present in at least 60% of the analysed microarrays were considered. The log2 ratio between the medians of the treated and control and the mean of the log2 ratio among the various arrays was calculated. Only LY2603618 溶解度 genes having a log2 ratio corresponding to at least 0. 5 fold over or under represented relative to control were considered differentially expressed, These data were finally statistically filtered using Acuity software T test analysis.